Uji Toksisitas Akut Ekstrak Air Dan Ekstrak Etanol Daun Kacapiring (Gardenia jasminoides Ellis) Terhadap Larva Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test (BSLT)

A toxicity test is a to detect the toxic effect of substance on biological system and obtain typical dose-response data from preparation. This study aims determine content secondary metabolites aqueous extract ethanolic gardenia leaves, characterization leaf extract, acute using BSLT method against Artemia salina Leach shrimp larvae.Phytochemical screening tests were carried out by color change which included alkaloid test, flavonoid tannin saponin terpenoid test. Extract include determination water-soluble ethanol-soluble water content, ash content. Acute Brine Shrimp Lethality Test (BSLT) larvae.The results phytochemical for leaves showed positive saponins, while ethanol extracts alkaloids, flavonoids, tannins, terpenoids. The that was 20.19%, 23.17%, 99.13% 0.29%. Meanwhile, in 60.80%, 57.14%, 7.87% 3.22%. show are not with LC50 value 1399.64 g/mL 1080.96 so it can be used as traditional medicine.